Figure 4
From: Modeling mutation-specific arrhythmogenic phenotypes in isogenic human iPSC-derived cardiac tissues
Modeling L/SQTS in 3D CTSs on MEA. (a) Generation and characterization of 3D CTSs. (b) Representative field potential waveform of 3D cardiac tissue sheet from gene edited hiPSCs (Parent vs SQT22 vs LQT26). (c) FPDcF quantification Parent/SQT/LQT (box plot, statistical analysis). Parent (n = 7), SQT22 (n = 5), LQT26 and LQT46 (n = 5), Dunnett’s test *P < 0.05, **P < 0.01 and ***P < 0.001. (d) Representative basal and arrhythmic field potential waveform of 3D CTSs in response to IKr blocker (E-4031) (Parent vs SQT22 vs LQT26). (e) Tendency of arrhythmogenicity in 3D cardiac tissue sheet (Parent (n = 13), SQT22 (n = 11), LQT26 (n = 11)). The denominator is the total number of samples, and the numerator is the number of samples in which the relevant arrhythmia phenomenon occurred. (f) Average of arrhythmogenic score in 3D cardiac tissue sheet (± s.e.m, Parent (n = 13), SQT22 (n = 11), LQT26 (n = 11), Dunnett’s test *P < 0.05).
