Table 3 Crystallographic parameters and refinement statistics.

	RT^3MB:DNA	RT^4M:DNA	RT^4M:DNA	RT^4M:DNA
Bound NA/dNTP	E-CFCP-TP	E-CFCP-TP	ETV-TP	dGTP
PDB code	8XZ1	8X20	8X21	8X22
Data collection
Beamline	PF BL-17A	PF BL-1A	PF BL-1A	PF BL-1A
Detector	Pilatus3S 6M	Eiger X4M	Eiger X4M	Eiger X4M
Wavelength (Å)	0.98000	1.10000	1.10000	1.10000
Space group	H3	H3	H3	H3
Unit-cell parameters (Å)	a = b = 283.1, c = 95.4	a = b = 284.1, c = 95.6	a = b = 284.4, c = 95.7	a = b = 285.2, c = 96.1
Resolution (Å)*	50–2.62 (2.67–2.62)	50–2.70 (2.75–2.70)	50–2.33 (2.37–2.33)	50–2.31 (2.35–2.31)
Unique reflections	85,655	78,960	123,410	127,828
R_meas*^,†	0.074 (0.896)	0.105 (0.878)	0.157 (0.875)	0.075 (0.926)
Mean I/σ (I)*	16.8 (2.1)	10.8 (1.7)	7.4 (2.1)	15.0 (2.1)
Completeness (%)*	99.9 (100.0)	100.0 (100.0)	100.0 (100.0)	100.0 (100.0)
Multiplicity*	5.1 (5.2)	5.5 (5.5)	5.5 (5.6)	5.5 (5.6)
Wilson B-factor (Å²)	61.2	63.3	49.3	48.0
Refinement
R_work/R_free^‡,§	0.179/0.223	0.173/0.231	0.176/0.218	0.182/0.219
No. of atoms	17,553	17,537	17,751	17,744
Average B-factors (Å²)
All/DNA	75.0/71.9	74.0/70.5	64.0/62.6	65.0/63.1
Ligand	103.4 (E-CFCP-TP)	111.2 (E-CFCP-TP)	72.2 (ETV-TP)	57.9 (dGTP)
R.m.s.d. from ideal
Bond lengths (Å)	0.007	0.004	0.012	0.008
Bond angles (°)	0.813	0.805	1.188	0.934
Ramachandran plot^¶
Favored	95.8	95.9	96.2	96.7
Outliers (%)	0.3	0.0	0.0	0.0

*Values in parentheses are for the outermost resolution shell.
^†R_meas = Σ_h Σ_i |I_h,i − < I_h >|/Σ_hΣ_i I_h,i, where < I_h > is the mean intensity of a set of equivalent reflections.
^‡R_work = Σ |F_obs − F_calc|/Σ F_obs for 95% of the reflection data used in the refinement. F_obs and F_calc are the observed and calculated structure factor amplitudes, respectively.
^§R_free is the equivalent of R_work, except that it was calculated for a randomly chosen 5% test set excluded from refinement.
^¶Ramachandran analysis was performed using MolProbity⁵⁰.

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