Fig. 2 | Scientific Reports

Fig. 2

From: A composite patch loaded with 2-Deoxy Glucose facilitates cardiac recovery after myocardial infarction via attenuating local inflammatory response

Fig. 2The alternative text for this image may have been generated using AI.

Blockage of glycolysis compromises the proinflammatory polarization of macrophages. (A–D) Relative gene expression of Nos2 (A), Il1b (B), Il6 (C), and Tnfa (D) in macrophages under OGD with or without 2-DG pretreatment (n = 3). (E,F) The iNOS protein expression of macrophages under OGD ± 2-DG (E) and the quantification of immunoblotting (F) (n = 3). Original blots/gels are presented in Supplementary Figure S5. (G,H) Representative flow cytometry analysis (G) and quantification (H) of the iNOS+ macrophages (n = 4). (I,J) Representative immunofluorescence images (I) and quantification of the iNOS (green) fluorescence intensity (J) (n = 6). Scale bar, 50 μm. Error bars represent the SD, and significance was determined using one-way ANOVA followed by Tukey’s test (*p < 0.05, **p < 0.01, and ***p < 0.001).

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