Fig. 6
Resveratrol protects HT-22 cells from the CM-induced mTORC1 activation and ER-stress. (A) Immunoblot analysis of p-p70, p70, p-ULK1 757, ULK1 using β actin as loading control, in the cell extracts of HT-22 cell lines exposed to conditioned media for 0, 8 and 24 h with and without Resveratrol 30 µM 4 h (n = 5). The plot indicates the quantification data of Mfn1, Mfn2, p-p70/p70, p-ULK1 757/ULK1 ratio under the different conditions. Data represent the mean ± standard error of the mean (SEM). *p < 0.05; **p < 0.01; ***p < 0.005 by the use of unpaired Student’s t-test. (B) Immunoblot analysis of BIP, p-eIF2α, eIF2α, p-PERK, PERK, using β actin as loading control, in the cell extracts of HT-22 cell lines exposed to conditioned media for 0, 8 and 24 h with and without Resveratrol 30 µM 4 h (n = 5) The plot indicates the quantification data of BIP, p-eIF2α/eIF2α, p-PERK/PERK ratio under the different conditions. Data represent the mean ± standard error of the mean (SEM). *p < 0.05; ***p < 0.005 by unpaired Student’s t-test.
