Fig. 2 | Scientific Reports

Fig. 2

From: Puerarin pretreatment provides protection against myocardial ischemia/reperfusion injury via inhibiting excessive autophagy and apoptosis by modulation of HES1

Fig. 2The alternative text for this image may have been generated using AI.

Pue inhibits A/R-induced oxidative stress and excessive autophagy in H9c2 cardiomyocytes. (A) Cell Counting Kit-8 assay of A/R-induced cells viability after Pue, RA or 3-MA pre-treatment. (B) LDH, (C) SOD, (D) MDA, (E) GSH-Px, (F) GSH/GSSG ratio, (G) Caspase-3 activity and (H-K) Protein expression of HES1, P62, and LC3II/I was determined using Western bloting analysis in cell lysates after A/R-induced following pre-treatment with Pue, RA or 3MA. Data are showed as the mean ± SD (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001. Pue, Puerarin; RA, rapamycin; 3MA, 3-methyladenine; A/R, anoxia/reoxygenation; SOD, superoxide dismutase; MDA, Malondialdehyde; GSH/GSSH, glutathione/glutathione disulfide; GSH-Px, glutathione peroxidase. HES1, Hairy and Enhancer of Split-1; P62, Sequestosome 1; LC3B, microtubule-associated protein 1 light chain 3; ns, not significant.

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