Fig. 4 | Scientific Reports

Fig. 4

From: Intratracheal instillation of RSV is superior to intranasal inoculation as a model method inducing critical immune cell infiltration

Fig. 4The alternative text for this image may have been generated using AI.

Comparison of RSV infected mice with two kinds of modeling methods. (A) The daily body weight changes of mice in the two RSV infection groups, the solid line is the dotted line of the average daily body weight of mice. (B) The virus titer was detected by immunofluorescence in mouse lung tissue. Plaques were counted in three randomly selected fields under a fluorescence microscope at 200× magnification, and the viral titer (PFU/g) was calculated as: (mean plaques/well × dilution factor⁻¹)/inoculum mass (g). The left picture shows the microscopic fluorescence result, and the right picture shows the calculated virus titer result. (C) RT-qPCR was used to detect the changes of RSV expression in lung tissue of RSV infected mice (n ≥ 4 mice in each group at each time point). (D) RT-qPCR was used to detect the changes of RSV expression in turbinate bone tissue of RSV infected mice (n ≥ 4 mice in each group at each time point). (E) RT-qPCR was used to detect the changes of RSV expression in tracheal tissue of RSV infected mice (n ≥ 4 mice in each group at each time point). *The degree of significance, *P < 0.05, **P < 0.01, ***P < 0.001.

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