Fig. 1
From: Development and in vitro characterization of humanized antibodies for blocking human TRPM4 channel
Characterization of the TRPM4 humanized antibodies. (A) HEK 293 cells were transfected with human TRPM4 (Hu) and mouse TRPM4 (Ms). Western blot was performed using the humanized antibodies Ab1-7. M4P was used as negative control and M4M as positive control. (B) HEK 293 cells transfected with human TRPM4 were incubated with control human IgG or Ab1-7 at a concentration of 20 µg/ml for 30 min before voltage ramps from − 100 to 100 mV were applied. The current voltage relationships of TRPM4 currents at 0 min baseline and after 7-min ATP depletion were recorded. N = 6. (C) Comparison of currents before and after 7-min ATP depletion at – 80 mV or 80 mV. # P < 0.0001, Student’s t test.
