Fig. 4
CypD promoted apoptosis pathway activation in DLD1 cells via autophagy regulation. (a) Western blotting analysis of apoptosis- and autophagy-related protein expression in DLD1 cells across experimental groups. All Western blots were derived from physically cut membrane strips, and lanes non-adjacent or from different gels were separated by white space. (b) Semi-quantitative analysis of apoptosis-related proteins (Bcl-2, Bax, and Cleaved-Caspase 3) following CypD knockdown or overexpression (independent-samples t-test). (c) Semi-quantitative assessment of autophagy-related proteins (p62, ATG7, ATG5, and LC3) expression under CypD modulation (independent-samples t-test). (d) Protein expression of Bax, Cleaved-Caspase 3, and LC3 in Ad vector and Ad CypD groups with or without autophagy inhibitor CQ treatment. All Western blots were derived from physically cut membrane strips, and lanes non-adjacent or from different gels were separated by white space. (e) Semi-quantitative analysis of corresponding protein expression from part d (one-way ANOVA followed by Tukey’s HSD test). * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001.
