Fig. 6
From: MiR-142-3p targets the CXCL12/WNT/β-catenin pathway to regulate the stemness of breast cancer cells
MiR-142-3p can regulate the migration, EMT and stemness of MCF 7 through affecting the WNT/β-catenin signaling pathway (a-b) The impact of various treatments on the migratory capability of MCF 7 cells was assessed using scratch and transwell assays. (c) Western blot analysis was conducted to examine the expression levels of E-cadherin protein and Snail protein across different treatment groups; (d) The effects of different treatments on the stemness of MCF 7 cells were observed through microsphere formation experiments; (e) Western blot analysis was utilized to investigate the regulatory effects of miR-142-3p inhibitor and ICG001 on the expression of stemness proteins in MCF 7 cells; (f) Immunofluorescence assays were employed to evaluate the effects of different treatments on the expression of β-catenin protein in MCF 7 cells. (*P < 0.05, **P < 0.01).
