Table 3 PCR-based markers for flowering time based on linkage mapping studies7,42,49 and allele count observed in white lupin germplasm panel.
Marker name | Polymorphism detection | Reference allele products (pb) | Alternative allele products (bp) | Reference allele count | Heterozygote count | Alternative allele count |
|---|---|---|---|---|---|---|
QTL01 (MFT-FT3-F1) | Product length | 295 | 311 | 231 | 22 | 47 |
QTL02 (FTc1-F4) | Product length | 298 | 291 | 76 | 8 | 216 |
QTL03 (FY-F6) | CAPS, Tsp45I | 233, 202 | 435 | 40 | 15 | 245 |
QTL05 (VIP3-F2) | CAPS, BspLI | 114, 85, 70 | 184, 85 | 45 | 21 | 234 |
QTL06 (TP23903) | CAPS, BseGI | 64 | 49, 15 | 121 | 25 | 143 |
QTL07 (TP235608) | CAPS, AflII | 217 | 179, 38 | 168 | 26 | 106 |
QTL08 (TP94353) | CAPS, RsaI | 60, 51 | 111 | 74 | 22 | 204 |
QTL09 (SKIP1-F2) | dCAPS, BseDI | 79 | 48, 31 | 291 | 3 | 6 |
QTL10 (TP402859) | CAPS, HpaII | 198 | 112,86 | 78 | 42 | 180 |
QTL11 (FTa1-F2) | Product length | 2218 | 1535 | 295 | 1 | 4 |
QTL12 (SEP3-F1) | dCAPS, TaqI | 122, 23 | 145 | 146 | 41 | 113 |
QTL13 (TP86766) | CAPS, DdeI | 64 | 48, 16 | 125 | 9 | 166 |
QTL14 (PIF4-F6) | CAPS, Hpy188III | 138, 52 | 102, 52, 36 | 204 | 17 | 79 |
QTL15 (TP47110) | CAPS, HpyF3I | 42, 24 | 66 | 299 | 0 | 1 |
QTL16 (TP345457) | CAPS, BseDI | 227, 39, 12 | 143, 84, 39, 12 | 233 | 24 | 43 |
