Table 1 PCR primers and amplification conditions.
From: Diversity and pathogenicity of pestalotioid fungi infecting Camellia oleifera in China
PCR primers (forward/reverse) | PCR amplification conditions | References |
|---|---|---|
ITS (ITS5/ITS4) | 94 °C: 5 min, (94 °C: 30 s, 52 °C: 50 s, 72 °C: 1 min) × 35 cycles, 72 °C: 7 min | White et al. (1990)22 |
tub2 (Bt-2a/Bt-2b) | 94 °C: 5 min, (94 °C: 30 s, 55 °C: 50 s, 72 °C: 1 min) × 35 cycles, 72 °C: 7 min | Glass et al. (1995)23 |
tef1-α (EF1-728 F/EF1-986R) | 94 °C: 5 min, (94 °C: 30 s, 55 °C: 50 s, 72 °C: 1 min) × 35 cycles, 72 °C: 7 min | Carbone et al. (1999)24 |
