Table 2 Summary of the experimental results reported in this manuscript.

From: Traceable stiffness calibration of colloidal AFM probes for biomechanical measurements

AFM probes

Reference cantilever

Colloidal probes

SD-R30-CONT, NanoSensorsTM

HQ: XSC11, \(\upmu\)Masch (glued microsphere, R = 20 \(\upmu\)m)

CP-CONT-BSG, (R = 10 \(\upmu\)m), sQube

CP-CONT-PM, (R = 7.5 \(\upmu\)m), sQube

Beam Dimensions, \(\upmu\) m

L

450

150

450

450

\(H_t\)

15

50

20

15

Stiffness ± 4%, N/m

\(k_m^{L}\)

0.394

5.718

0.366

0.487

\(k_m^{U}\)

0.394

5.129

0.360

0.470

\(k_c\)

0.394

5.408

0.362

0.478

Friction coefficient, \(\mu\)

 

\(\mu _{\mathrm{Si-Diamond}}\) = 0.00

\(\mu _{\mathrm{Glass-Ruby}}\) = 0.11

\(\mu _{\mathrm{Glass-Diamond}}\) = 0.02

\(\mu _{\mathrm{{PMMA-Diamond}} }\) = 0.04

Stiffness by TU Ilmenau, N/m

\(k_m^{EMFC}\)

0.3930 ± 0.0046

na

0.3570 ± 0.0055

na

\(k_m^{ESFC}\)

0.3968 ± 0.0022

na

0.3568 ± 0.0016

na