Table 4 A comparative overview of synthesis and characterization of silver nanoparticles with their significance.

From: Artificial intelligence tools for AgNP-SSB-SN and AgNP-CSS-SN biosynthesis from Synechococcus PCC 11901 and Chlorella sorokiniana MSP1 for hazardous dyes remediation

Microalgae/Cyanobacteria/ Algae

Optimization techniques used

Conditions during optimization

Characterizations

Significance

Reference

FESEM

XRD

TEM

PDI

Zeta potential

Chlorella vulgaris

OFAT

Extract ratio- 8:2 v/v, pH-12, 3 mM AgNO3 and 24 h of incubation time

55.06 ± 9.67 nm

61.89 ± 12.13 nm in crystalline nature

-

0.56

– 26.4 ± 4.22 mV

Showed significant photocatalytic dye degradation activity of methylene blue

33

Chlorella minutissima (aqueous extract)

OFAT

pH-9, AgNO3: extract ratio- 1:10,

Spherical in shape

(200 nm)

-

73.13 nm

0.25

– 21.2 mV

Showed significant growth inhibition of gram positive and gram-negative bacterial species

68

Chlorella vulgaris

In-situ photocatalysis

Temperature- 85 ℃, 0.1 M AgNO3, pH-8, Incubation time- 1.5 h

500 nm

24.61 nm crystalline nature

-

-

-

90.6% of Brilliant Blue R degradation was achieved after 90 min of irradiation

69

Planophila laetevirens

OFAT

1:4 v/v of algal extract to silver nitrate, Temperature- 80 ℃, Dark incubation

Quasi-spherical

(200 nm)

-

10.8 ± 0.3 nm

0.56

– 26.1 mV

Significant antitumor activity against colon and breast cancer

70

Chlamydomonas reinhardtii (EPS)

OFAT

Concentration- 1.25 mM, Incubation time- 24 h, light required

Spherical

-

7.5 nm

-

To achieve the scalability of EPS-PBR- wastewater systems

71

Nostoc linckia

RSM and FCCCD

pH-10, AgNO3

Concentration- 5 mM, Incubation time- 24 h, Pigment concentration- 1 mgmL-1

Crystalline

3.24 nm

9.39 to 25.89 nm

– 31.8 mV

Showed efficient antibacterial, anticancer and antihemolytic activities

72

Haematococcus pluvialis (Cell-free supernatant)

OFAT and Pseudo- first-order kinetic

Temperature- 45 ℃, pH- 11, AgNO3 to CFS ratio v/v- 90:10, stirring- 180 rpm for 15 min followed by stirring at 80 rpm

Face-centered cubic structure

30–50 nm

0.22

– 35.9 to -18.2 mV

Demonstrated the antibacterial activity against E. coli

73

Synechococcus sp. PCC 11901 and

Chlorella sorokiniana MSP1

RSM and ANN-GA hybrid tool

For SSB-SN: Bioextract- 2.49 mL, AgNO3 concentration- 2.42 mM, Incubation time- 22.78 h

For CSS-SN: Bioextract- 2.49 mL, AgNO3 concentration- 8.07 mM, Incubation time- 19.37 h

SSB-SN appeared as cubic structure

CSS-SN appeared as spherical structure

Both AgNPs show crystalline structure

12.61 nm

26.60 nm

0.22

0.37

– 20.95 mV

– 31.17 mV

SSB-SN degraded the orange-II and Sudan black dyes up to 99.14% and 98.16%

CCS-SN demonstrated around 73.16 and 97.75% for orange-II and Sudan black dyes, respectively

This study

  1. FESEM: Field emission scanning electron microscopy; XRD: X-ray diffraction; TEM: Transmission electron microscopy; PDI: Polydispersity index; OFAT: One factor at a time; RSM: Response surface methodology; FCCCD: Face centred central composite design; ANN-GA: Artificial neural network-genetic algorithm.