Fig. 5
From: Change in the chondroitin/dermatan structure in distal lung tissue from COPD patients
Effect of TGF-β activity. (A) Gene set enrichment analysis (GSEA) of ranked list generated from differential expression of RNAseq data from cohort 2 was performed on COPD patients (GOLD stage I-II, n = 96) versus controls (n = 91) lung tissue enriched against cellular response to TGF-β stimulus. (B) Pearson and Spearman correlation between CHST11 and TGF-β score generated using enrichment scoring for each sample. Correlation of all GAG enzymes are found in Supplemental Fig. S5. (C) Quantification of proteoglycans in HFL1 cells following TGF-β stimulation measured using SRM technology with pre-chosen peptides described in supplemental Table 1, (n = 4). (D) Quantification of GAGs in HFL1 cell medium following TGF-β stimulation (n = 4). (E) Quantification of HS disaccharides in HFL1 cell medium following TGF-β stimulation (n = 4). (F) Quantification of CS/DS disaccharides in HFL1 cell medium following TGF-β stimulation, (n = 4). (G) Quantification of DS disaccharides in HFL1 cells following TGF-β stimulation using AMAC-labelling after chondroitinase B digestion (n = 4). (H) mRNA fold change of selected CS/DS biosynthetic enzymes in TGF-β stimulated HFL1 cells compared to vehicle control measured by qPCR, (n = 4). Data is presented as mean ± S.D., *p < 0.05, **p < 0.01 and ***P < 0.001 using Student’s T-test.
