Fig. 4: Sensitivities of a mixture population of BaF3/RET(D898_E901del)/ETV6-NTRK3 and BaF3/EML4-ALK cells to selpercatinib, larotrectinib, entrectinib, and combination treatments.
From: Adaptive Darwinian off-target resistance mechanisms to selective RET inhibition in RET driven cancer
a an equal number of two cell lines were mixed, cultured in 96-well plates and treated with indicated drugs for three days, and viable cells were measured using CellTiter-Glo reagent. a. Drug response curves. b Cells were treated with indicated drugs for 4 h (kinase inhibition analyses) or 24 h (apoptosis analyses). Cell lysates were analyzed by immunoblotting with indicated antibodies.
