Fig. 2: Single-cell sequencing indicates cardiomyocyte heterogeneity.
From: Single-cell transcriptomics following ischemic injury identifies a role for B2M in cardiac repair
a tSNE plot indicating transcriptomic similarities across cardiomyocytes obtained from all conditions. Different colors, symbols and numbers highlight the clusters as determined by k-medoids clustering of 1 − Pearson’s correlation coefficient. b Bar graph showing the proportion of cardiomyocytes originating from the different condition per cluster. c Heatmap showing expression of all genes significantly enriched in at least one cluster. Examples of genes enriched per cluster are depicted on the right side of the heatmap. d Bubble plot of top GO terms in gene ontology analysis on genes significantly enriched within the cluster. b–d Numbers highlighted in red depict a cluster that mainly contains 3 dp IR and 14 dp IR cardiomyocytes (cluster 5). e, f Cell trajectory analysis of cardiomyocytes, showing pseudotime on a color-coded scale (e) or highlighting the k-medoids cluster of each cardiomyocyte in the trajectory plot (f). The regions in the branches of the trajectory plot that contain mostly cells from k-medoids clusters 1,2,3 or cluster 4 or cluster 5 are highlighted by number.
