Fig. 3: Metastatic ability of a mixed cancer cell population of TGF-β-stimulated and unstimulated cells.
From: Whole-organ analysis of TGF-β-mediated remodelling of the tumour microenvironment by tissue clearing
Analysis of the cooperative metastasis between the TGF-β-stimulated cancer cells and the unstimulated cancer cells. A549-mCherry or A549-GFP cells were pre-stimulated with or without TGF-β1 for 3 days, respectively, and intravenously injected in mice at the indicated number (day 0). Then, the lung was subjected to whole-organ clearing protocol (day 14), followed by 3D imaging. a Representative images. b Quantification of the metastatic colony number and the total metastatic tumour volume of A549-GFP cells in the lungs of mice. c Quantification of the metastatic colony number and the total metastatic tumour volume of A549-mCherry cells in the lungs of mice. 3D image (whole), scale bar = 2000 μm. 2D image, scale bar = 200 μm. Mouse number in each group is n = 5. Representative result of two independent experiments. Data represent the effect size as a bootstrap 95% confidence interval.
