Fig. 2: Cephalic response to oral high fat and gastric secretions in germline Cd36^−/− mice.

a–c Impaired response of plasma leptin, insulin, and pancreatic polypeptide in fasted mice and 15 min after an oral high fat meal. d Stomach acetylcholine levels in 4 h refed mice. e,f Gastric secretions at 1 h after pylorus ligation in 18 h fasted mice given i.p. saline (Control), carbachol (CCh, 60 µg/kg) or histamine (H, 10 mg/kg). e Gastric juice pH. f Immunoblots of gastric juice; quantification of gastric intrinsic factor (GIF)/total protein in juice showing reduced GIF output in Cd36^−/− mice given carbachol. g Chief cells markers by qPCR and immunostaining (green) in corpus of wildtype (WT) and Cd36^−/− mice, scale: 50 µm. h mRNA of muscarinic receptor 1 (M1r) in the corpus of WT and Cd36^−/− mice. Significance was calculated using a two-way ANOVA followed by post hoc tests by Sidak multiple comparisons or a two-sided unpaired t test (d and h). *P < 0.05, **P < 0.01. a–c n = 4–5 per condition/group; d: n = 7–12; e,f n = 3–4; g,h n = 5.