Fig. 3: Helix angle analysis and RNA-FISH imaging and quantification of exercise and sedentary mouse hearts.

a Helix angles change from the endocardium to epicardium between sedentary and exercised mice. b A visual representation of the location of the six AHA sections and a zoomed in images of the complete cross-section across each transmural layer. c Complete image of sectioned mouse tissue with AHA sections overlayed. d CITED4 signal isolated within mid-myocardium. e DAPI signal isolated and magnified in the mid-myocardium. f CITED4 and DAPI signal imaged from mid-myocardium overlayed.