Fig. 2: 2D, 3D, and ex vivo culture from fresh and slow-frozen matching tumor samples. | Communications Biology

Fig. 2: 2D, 3D, and ex vivo culture from fresh and slow-frozen matching tumor samples.

From: Live slow-frozen human tumor tissues viable for 2D, 3D, ex vivo cultures and single-cell RNAseq

Fig. 2: 2D, 3D, and ex vivo culture from fresh and slow-frozen matching tumor samples.The alternative text for this image may have been generated using AI.

a Percentage of successfully established melanoma cell lines from fresh or slow-frozen biopsies. b Representative bright-field micrographs of a successful melanoma cell line and a cell line contaminated with non-melanoma cells. Scale Bar 100 μm and 30 μm (insert). c Microscopic photographs of matched colon carcinoma primary parental tumor tissue and organoids derived from fresh and slow-frozen tissues. Scale bar lengths are 100 μm, for insert microscopic photographs 50 μm. CK20: Keratin 20, PAS-AB: Periodic acid-Schiff Alcian Blue, Ki67. d Microscopic photographs of shave biopsies from matched fresh or slow-frozen BCC stained with Hematoxylin-eosin (H&E) BErEp4 and Ki67 antibodies. Scale bar lengths are 2 mm and 200 μm.

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