Fig. 4: Correlation and differential gene expression analysis of fresh and slow-frozen scRNAseq tumor samples.
From: Live slow-frozen human tumor tissues viable for 2D, 3D, ex vivo cultures and single-cell RNAseq
a UMAP visualization of fresh and slow-frozen samples according to the preservation method. b Boxplot showing the correlations between the gene expression of cells in fresh samples of different patients (purple) and the correlations between the gene expression of cells of fresh vs frozen samples (orange). Dots depict the correlation value for each sample pairing (e.g., Sample1_fresh vs Sample2_fresh or Sample1_fresh vs Sample1_frozen). c Heatmap showing differentially expressed genes between fresh and frozen samples (genes differentially expressed in at least 9 out of the 18 cell types). d Negative log10 p values of the enrichment analysis for the Reactome pathway “Cellular response to heat stress” for each cell type.
