Fig. 2: AtCHE1 encodes AATF/CHE-1 in Arabidopsis. | Communications Biology

Fig. 2: AtCHE1 encodes AATF/CHE-1 in Arabidopsis.

From: AtCHE1, the Arabidopsis homolog of mammalian AATF/Che-1 protein, is involved in safeguarding genome stability

Fig. 2: AtCHE1 encodes AATF/CHE-1 in Arabidopsis.The alternative text for this image may have been generated using AI.

A Sequence comparison of the wild type (AtCHE1) and mutant (che1) DNA, CDS, and predicted proteins. The red frame shows the last base of the eighth intron, in which G is mutated to A by EMS mutagenesis. The green frame and black frame show the missing sequence ‘AACGTTAG’ and the formation of a stop codon by mis-splicing. The green arrow indicates the last amino acid of the predicted che1 mutant protein. The CDS sequences were obtained by reverse transcription-polymerase chain reaction (RT-PCR) using total RNA isolated from mutant and wild-type seedlings. B Comparison of the conserved protein boxes between the human AATF/Che-1 (hAATF) and AtCHE1. Light blue box: AATF/Che-1 domain, lila box: TRAUB, orange box: Nuclear localization signal (NLS), yellow box: Nucleolar localization sequence (NoLS). Red arrowhead: position of the last amino acid of che1. Blue letters and numbers indicate the potential phosphorylation sites on serine and threonine residues in hAATF and AtCHE1, respectively.

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