Fig. 3: AtCHE1 is widely expressed and localized in nucleus.

A–G represent GUS expressional studies in A young seedling, B root, C young shoot, D enlarged image of mature rosette leaf, E mature rosette leaf, F flowers and G silique. The white arrowhead in (A) points to the root apical meristem. H The representative confocal image illustrates the expression of AtCHE1 in the root meristem followed in the AtCHE1_pro:AtCHE1_g-GFP line (6 DAG). The enlarged section highlights its reduced level of expression in the QC compared to the surrounding stem cells. Magenta: PI staining; green, GFP fluorescence. Arrowheads (White) point to QC and the first elongated cortex cell (Green). Scale bars =1000 µm in (A, C, D–G), scale bars = 50 µm in (B) and (H). I Confocal images of the nuclear localization of the AtCHE1-GFP in the AtCHE1_pro:AtCHE1_g-GFP line transformed with the 35 s:H2B-tdTomato marker. Magenta: H2B-tdTomato; green, AtCHE1-GFP. Scale bar = 5 µm. J DAPI-stained nuclei of the AtCHE1_pro:AtCHE1_g-GFP line. Blue: DAPI; green, GFP. Scale bar = 5 µm. K and L Gray value distribution of the total number of pixels at the position of the white lines marked in (I) and (J).