Fig. 4: Auxin signaling and expression patterns of root developmental regulator genes are altered in che1. | Communications Biology

Fig. 4: Auxin signaling and expression patterns of root developmental regulator genes are altered in che1.

From: AtCHE1, the Arabidopsis homolog of mammalian AATF/Che-1 protein, is involved in safeguarding genome stability

Fig. 4: Auxin signaling and expression patterns of root developmental regulator genes are altered in che1.The alternative text for this image may have been generated using AI.

A represents the expression of DR5rev:GFP. B of PLT1pro:CFP and C PLT2pro:CFP in the root tips of 5 DAG Col-0 and che1. D Expression pattern of SCRpro:GFP and E SHRpro:SHR-GFP in the root tips of 5 DAG Col-0 and che1. A–E In each image, scale bar = 50 µm; arrowhead points to QC, Magenta, PI staining; Green, GFP, Cyan, CFP. F Relative mRNA levels of PLT1 and PLT2 quantified by qRT-PCR in Col-0 and che1. G SCR and SHR relative mRNA levels (gene vs reference gene) determined by qRT-PCR in Col-0 and che1. For the analysis, both in (F and G), root tips (2 mm long) were collected, values and error bars represented means and ±SD from three independent biological replicates. Asterisks indicate significant differences compared with Col-0. (**P value < 0.01, *P value < 0.05, two-tailed Student’s t test).

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