Fig. 7: Loss of AtCHE1 partially rescues impaired root development of atr-2 under HU treatment. | Communications Biology

Fig. 7: Loss of AtCHE1 partially rescues impaired root development of atr-2 under HU treatment.

From: AtCHE1, the Arabidopsis homolog of mammalian AATF/Che-1 protein, is involved in safeguarding genome stability

Fig. 7: Loss of AtCHE1 partially rescues impaired root development of atr-2 under HU treatment.The alternative text for this image may have been generated using AI.

A, B Representative images of root meristems of 6 DAG Col-0, che1, atr-2 and atr-2;che1. Seedlings grown on 1/2 MS medium with or without 1 mM HU. Scale bars = 50 µm; arrowheads point to QC. C Measurement of root meristem length and D root meristem cell number of 6 DAG Col-0, che1, atr-2 and atr-2;che1. Seedlings grown on 1/2 MS medium with or without 1 mM HU. E and F Confocal images of PI-stained root tips of Col-0, che1, atr-2 and atr-2;che1 double mutants. 4 DAG seedlings were transferred and grown on 1/2 MS medium with or without 2 mM HU for 24 h. Scale bars = 50 µm; arrowheads point to QC. G Measurement of the percentage of roots showing dead cells. H Measurement of dead cell area from Col-0, che1, atr-2 and atr-2;che1 PI-stained roots. The values and error bars in (C, D) and (G, H) represent means and ±SD from three independent experiments with at least 8 seedlings each. Columns with different letters indicate significant differences at P < 0.05 (Duncan’s multiple range mean comparisons).

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