Fig. 2: CCDC141 is highly expressed in cardiomyocytes.
A Gene structure of CCDC141. The human CCDC141 gene spans 220 kb, contains 24 exons, and is located upstream of the connectin gene. An RNA transcript spanning the CCDC141-TTN region has been identified (Labeit et al., 200610). ‘M’ indicates the initiating methionine at the start codon, while ‘S’ marks a position where a stop codon is present. B Domain structures of CCDC141 isoforms. Boxes indicate spectrin repeats (purple), coiled-coil motif (ocher), Ig domain (red), and transmembrane domain (black). Bar indicates epitopes recognized by CCDC141-N and CCDC141-C antibodies. C Quantitative PCR analysis of CCDC141 expression across mouse tissues. CCDC141 is highly expressed in the heart. Forward primers target exon 21 and reverse primers target exon 22. Data are presented as mean ± SE. n = 3. Endogenous control: 18S rRNA. D Western blot detection of CCDC141 across mouse tissues using CCDC141-N and CCDC141-C antibodies. Asterisks indicate bands resulting from non-specific antibody binding. E Western blot analysis of CCDC141 expression in embryonic (E17), postnatal (P0), and adult hearts using CCDC141-N and CCDC141-C antibodies. F Immunofluorescence microscopy showing CCDC141 localization in mouse embryonic (E17) myocardium, using CCDC141-N and CCDC141-C antibodies and counterstained with α-actinin antibody and DAPI. Arrowheads indicate perinuclear localization, while arrows indicate the Z-lines. Boxes mark regions used for Merge ×4 magnified images. G Immunofluorescence microscopy of CCDC141 localization in adult mouse myocardium using CCDC141-N and CCDC141-C antibodies, counterstained with α-actinin antibody and DAPI. Arrows indicate the costameres. Boxes mark regions used for Merge ×4 magnified images.
