Fig. 1: Differential formation of p62 bodies and stress granules in response to hyperosmotic stress. | Communications Biology

Fig. 1: Differential formation of p62 bodies and stress granules in response to hyperosmotic stress.

From: Rapid activation of p62 body-mediated autophagy in human cells under hyperosmotic stress

Fig. 1: Differential formation of p62 bodies and stress granules in response to hyperosmotic stress.The alternative text for this image may have been generated using AI.

A Immunostaining of T24 cells exposed to hyperosmotic stress using antibodies against p62 (PM045) and G3BP1. Cells were cultured under normosmotic conditions without medium exchange (No Me), or shifted to DMEM containing indicated concentrations of sucrose for 20 min. Boxed regions are enlarged and shown as insets. Scale bars = 20 µm and 2 µm (insets). B Quantification of the number of p62 bodies and stress granules in T24 cells and U2OS cells (images are shown in Supplementary Fig. 1A). The number of cytoplasmic p62 (green) or G3BP1 (red) puncta per area was quantified in 45 cells from three independent experiments. Data are presented as mean ± SD. Each value was statistically compared to “0.1 M” group using one-way ANOVA followed by Tukey’s test. ** P < 0.01, *** P < 0.001, NS: not significant. C Quantification of the size of p62 puncta in T24 cells and U2OS cells. A minimum of 150 puncta was measured from three independent experiments. Data are presented as mean ± SD. Each value was statistically compared to “0.1 M” group using one-way ANOVA followed by Tukey’s test. *** P < 0.001, NS not significant.

Back to article page