Fig. 4: Ultrastructural analysis of p62 bodies and stress granules using CLEM.
From: Rapid activation of p62 body-mediated autophagy in human cells under hyperosmotic stress
A CLEM images of U2OS cells stably expressing GFP-G3BP1 and mCherry-p62, cultured in DMEM with 0.3 M sucrose for 20 min. CLEM images (fluorescence, EM, and merged images) are shown on the left, with the regions (ii) and (iii) enlarged below, as indicated. The region (sup) is shown in Supplementary Fig. 4A. The black-flamed EM image is enlarged on the right (iv); the boxed regions are further magnified below (v). Red arrows and green arrowheads indicate p62- and G3BP1-positive structures, respectively. B CLEM images of ARPE-19 cells cultured in DMEM with 0.3 M sucrose for 20 min and immunolabeled using anti-p62 (PM045) and anti-G3BP1 antibodies. The layout is the same as (A). The region (sup) is shown in Supplementary Fig. 4B. Red arrows and green arrowheads indicate p62- and G3BP1-positive structures, respectively. Scale bars = 20 µm (i), 5 µm (ii), 1 µm (iii and iv), 200 nm (v).
