Fig. 2: Plxdc2-GFP+ cells are highly enriched for functional HSCs.
From: Prospective isolation of mouse and human hematopoietic stem cells using PLXDC2
A Experimental design. B Chimerism kinetics of donor cells in the indicated lineages. Source data are provided as a Supplementary Data file. C Expression profiles of Lin, c-Kit, Sca-1, CD34, and CD150 on Plxdc2-GFP+ whole BM cells and Plxdc2-GFP- whole BM cells. D Representative imaging of femoral BM slice from Plxdc2+/GFP mice stained with CD150, lineage markers, CD48, and CD41. Plxdc2-GFP⁺ cells (green) and CD150⁺ cells (red) are shown, revealing substantial spatial overlap. CD150⁺ cells (red) are co-stained with lineage markers (Lin, cyan), CD48 (cyan), and CD41 (cyan), enabling identification of SLAM-defined HSCs (CD150⁺Lin−CD48−CD41−). High-magnification insets of the yellow boxed region demonstrate a cell that is simultaneously Plxdc2-GFP⁺ (green), CD150⁺ (red), and negative for lineage, CD48, and CD41 (cyan), confirming that Plxdc2-GFP⁺CD150⁺ cells overlap with SLAM-defined HSCs. Scale bars = 50 µm and 10 µm (magnified images). Data are shown as the mean ± SEM.
