Fig. 1: Neto-mediated modulation of GluK3 receptor desensitization kinetics and rectification.
From: Multilayered regulation of GluK3 kainate receptors is mediated by Neto subunits and zinc
A Representative whole-cell current traces evoked by 30 mM glutamate (100 ms application) from HEK293 cells expressing GluK3 alone or co-expressed with Neto1 or Neto2. The inset shows a magnified view of the traces to facilitate visualization of differences in current kinetics (all normalized traces are shown using the same time scale). B Comparison of mean weighted desensitization time constants (τ des, ms) illustrated for whole-cell current traces (GluK3 n = 8, GluK3+Neto n = 11, GluK3+Neto2 n = 9) shown in (A). C Mean I-V relationships for GluK3 (n = 4) expressed alone or with Neto1 (n = 4) or Neto2 (n = 5) in the presence of 30 mM glutamate. GluK3 alone exhibits negligible outward currents, whereas co-expression with Neto1 or Neto2 produces prominent outward currents (inset, all normalized traces are shown using the same time scale). Whole-cell currents were normalized to the peak amplitude at –90 mV to allow comparison of rectification differences between depolarized and hyperpolarized potentials. D Pooled data of rectification index (RI, +90 mV/−90 mV) calculated from the corresponding I–V curves. Data are presented as mean ± s.e.m. Statistical significance was assessed using an unpaired two-tailed t-test (*p < 0.05;**p < 0.01; ***p < 0.001;****p < 0.0001).
