Fig. 6: Zinc potentiation of GluK3(D759G) desensitization is differentially modulated by Neto subunits.
From: Multilayered regulation of GluK3 kainate receptors is mediated by Neto subunits and zinc
A–C Mean weighted τdes of GluK3(D759G) alone (n = 9) and modulated by Neto1 (n = 5) or Neto2 (n = 6) currents were shown for 30 mM glutamate application and 100 μM Zn, along with 30 mM glutamate. In each panel, an inset shows normalized currents as a representation. All normalized traces are shown using the same time scale. D. Normalized histogram presents the effect of 100 μM zinc on the desensitization kinetics of GluK3(D759G) receptors in the presence of Neto1 or Neto2. E–G Peak glutamate-evoked current amplitudes recorded from cells expressing GluK3 alone (n = 9) or with Neto1 (n = 5) or Neto2 (n = 8). In each panel (above), the inset shows normalized currents as a visual representation. Neto2 had a significant facilitatory effect on GluK3(D759G)-mediated currents. H Histogram displays the normalized peak amplitudes from GluK3 (D759G) receptors with and without Neto proteins. Data are shown as mean ± s.e.m. Statistical significance was assessed using a paired two-tailed Student’s t test for comparisons in (A, B, E–G), and an unpaired two-tailed Student’s t-test for comparisons of normalized mean weighted desensitization time constants (τdes). Significance is indicated as *p < 0.05; **p < 0.01;***p < 0.001;****p < 0.0001; ns not significant.
