Fig. 3: FcRn and ion-exchange chromatography analysis of FcRn affinity engineered mutants.

a FcRn affinity chromatography of FcRn affinity engineered mutants. b Overall view of the ion-exchange chromatography. c A zoomed-in view of the ion-exchange chromatography. Solid lines represent the chromatogram of absorbance at 280 nm. The pH of elution is shown on top.