Table 1 Oligos used to amplify the diverse fragments for Gibson assembly to generate the GW-pcDNA5/FRT/TO-mEGFP-PARP15.1-T2A-3xFLAG-NLS-NBGFP construct
From: Screening assay to monitor mono-ADP-ribosylhydrolase activity of viral macrodomains in cells
Primer 1_fwd | ttagtgaaccgtcagatccgatgGACTACAAAGACCATGAC |
Primer 1_rev | CACCTTCCTCTTTTTCTTAGGCTTGTCATCGTCATCCTTG |
Primer 1a_rev | CTGCACCTGCATGGTGGCGAgacctccactgccCACCTTCCTCTTTTTC |
Primer 2_for | ccggactctagcgtttaaacgccaccatggtgagcaag |
Primer 2_rev | GCCTGGCGCAGCCATCGAAGCTTGAGCTCG |
Primer 3_for | CGAGCTCAAGCTTCGATGGCTGCGCCAGGC |
Primer 3_rev | cagggagccgcggccctcAGCCGTGAAAGTTATG |
Primer 3a_rev | CTCCACGTCGCCGCAGGTCAGcagggagccgcggccctc |
Primer 4_for | gagaaccccggccccatgGACTACAAAGAC |
Primer 4a_for | CTGACCTGCGGCGACGTGGAGgagaaccccggcccc |
Primer 4_rev | CAAACTTGTGATAGCGCTGGATACTGTCAC |
