Extended Data Fig. 2: Immunostaining with human cell marker and islet enriched transcription factors in hPSC-islet grafts in three extraperitoneal transplantation sites at 1 week post-transplantation.
a, Representative immunohistochemical staining of human cell marker Stem121 in human grafts in Monkey 1 and 2, which results showed the morphology and structure of residual human grafts in three tested transplantation sites at 1 wpt. Scale bars, 400 μm (Monkey 1) and 200 μm (Monkey 2); for the enlarged view (indicated with green border), 50 μm (Monkey 1) and 25 μm (Monkey 2). Similar results were obtained from five independent grafts. b-c, Representative immunofluorescence staining of C-peptide and β cell specific transcription factor PDX1 (b) or NKX2.2 (c) in human grafts in Monkey 1. Scale bar, 50 μm. Similar results were obtained from five independent grafts. d, Quantitative assessment of the expression of islet-enriched transcription factors (PDX1, NKX6.1 and NKX2.2) in human grafts in three indicated sites (n = 5 independent grafts). Data presented as mean ± SEM. Two-tailed t-test was used to determine significance between groups and P value was indicated above the bars.
