Fig. 5: PGC-1α is indispensable for the normal transcriptional response to acute maximal exercise.
From: Molecular control of endurance training adaptation in male mouse skeletal muscle
a, Bar Venn diagram of the genes altered in unperturbed trained WT (grey) and mKO (blue) muscle. b, All functional annotation clusters of genes that are only up- (orange) and downregulated (blue) in trained muscle of WT animals (up: n = 96; down: n = 147) with an enrichment score >2. c, Motif of the transcription factors from ISMARA with the most significant activity change in trained WT animals and the comparison of the activity in trained mKO muscle (left blue), gain-of-function model (sedentary muscle-specific PGC-1α transgenics (mTG), purple) and loss-of-function model (sedentary mKO, dark blue). d, Number of genes that are up- and downregulated 0, 4, 6 and 8 h after an acute maximal exercise bout in untrained WT (light grey), trained WT (dark grey), untrained mKO (light blue) and trained mKO (dark blue) animals. e,f, Examples of gene trajectories with the peak expression immediately post-exercise (e) or at a later time (f) in untrained WT and mKO animals. g, Venn diagrams of all up- and downregulated genes after an acute bout of exercise in untrained WT (light grey) and mKO (light blue) mice. h, All functional annotation clusters of up- (orange) and downregulated (blue) genes that are regulated only in untrained WT mice (745 genes up- and 314 genes downregulated) with an enrichment score >2. i, Examples of genes involved in ECM organization, microglial cell proliferation and Wnt signalling that are regulated only in WT muscle. j, Prediction of the activity of a motif using ISMARA that is changed only in WT muscle and might be involved in the regulation of ECM-related genes. The data are from five biological replicates and represent mean ± s.e.m (if not otherwise stated). Statistics of RNA-seq data were performed using the CLC Genomics Workbench Software. Exact FDR values of RNA-seq data and z-scores of ISMARA data are displayed in Source data. The asterisk indicates difference to Ctrl (pre-exercise condition): *P < 0.05 (for motif activity: *z-score > 1.96); **P < 0.01; ***P < 0.001 (Extended Data Fig. 7 and Supplementary Tables 4 and 8).
