Fig. 1: DDHD2 lipase localizes to synaptic terminals in the hippocampus.
From: Triglycerides are an important fuel reserve for synapse function in the brain
a, Schematic of a coronal section of rodent brain highlighting the hippocampus. The CA3 region is marked with a yellow box in the hippocampus. b, Laser scanned confocal micrograph of a brain hemisphere section near the mid-brain region, spanning the hippocampus, derived from a 28-day-old juvenile mouse brain, immunolabelled with DDHD2 and synapsin (synaptic terminal) antibodies. Scale bar, 400 µm. c, Confocal micrograph of the CA3 region (yellow box in b) of the hippocampus derived from a 28-day-old juvenile mouse brain, immunolabelled with synapsin (synaptic terminal) and DDHD2 antibodies. Scale bar, 50 µm. The lower panel shows the zoomed-in region (yellow box in upper right panel) of CA3. Scale bar, 10 µm. d, Confocal micrographs of dissociated hippocampal neurons immunolabelled with DDHD2 and synapsin antibodies. Scale bar, 20 µm. The lower panel shows the zoomed-in region (yellow box in upper right panel). Scale bar, 5 µm. e, Confocal micrographs of dissociated hippocampal neurons expressing GFP–DDHD2 and mRuby–synapsin. Scale bar, 10 μm. f, Quantification of relative DDHD2 and synapsin enrichment at synaptic terminals compared to adjacent axonal processes of hippocampal neurons in d. Data are presented as means; error bars, s.e.m. P value (ns, P = 0.501) was determined using an unpaired sample two-tailed t-test with n = 69 for DDHD2 and n = 64 for synapsin. g, Colocalization analysis of immunolabelled DDHD2 and synapsin (65 µm × 65 µm, n = 10), and GFP–DDHD2 and mRuby–synapsin (135 µm × 135 µm, n = 7) in hippocampal neurons, represented by Pearson’s correlation coefficient. Data are presented as means; error bars, s.e.m. Hipp., hippocampus; Immuno., immunostaining (of DDHD2 and synapsin); OE, overexpression (of GFP–DDHD2 and mRuby–synapsin).
