Extended Data Fig. 2: Subcellular localization of DDHD2.
From: Triglycerides are an important fuel reserve for synapse function in the brain
a, Confocal micrographs of hippocampal neurons treated with KLH45, immunostained for DDHD2, and stained with BODIPY to visualize lipid droplets. b-d, Confocal micrographs of hippocampal neurons co-immunostained for DDHD2 and organelle-specific markers: TOMM20 (mitochondria), catalase (peroxisomes), and ERGIC3 (ER-Glogi intermediate compartment). Scale bars: 10 μm; Zoomed-in panels: 1 μm. e, Confocal micrographs of hippocampal neurons expressing GFP-DDHD2 and TGNP-mCherry, showing their subcellular distribution. f, Confocal micrographs of hippocampal neurons expressing GFP-DDHD2 and stained with LysoTracker-Red to visualize lysosomes. Scale bars: 5 μm; Zoomed-in panels: 1 μm. Line-profile intensity graphs shown on the right correspond to the yellow lines drawn in the zoomed-in panels.
