Extended Data Fig. 3: Effect of DDHD2 and ATGL lipase inhibition on lipid accumulation in neurons and astrocytes.
From: Triglycerides are an important fuel reserve for synapse function in the brain
a, Confocal micrographs of control neurons (green, NeuN) and astrocytes (red, GFAP) stained with MDH/AUTODOT (cyan, lipid droplets). Scale bar: 20 μm. b, Confocal micrographs of KLH45 (DDHD2 inhibitor)-treated neurons (NeuN) and astrocytes (GFAP) stained with MDH (lipid droplets). Scale bar: 20 μm. c, Confocal micrographs of atglistatin (ATGL inhibitor)-treated neurons (NeuN) and astrocytes (GFAP) stained with MDH (lipid droplets). Scale bar: 20 μm. Lower panels (in a-c) show zoomed-in views of the upper panels (marked with yellow boxes). Scale bars: 10 μm. d, Quantification of LD numbers in hippocampal neurons following KLH45 and atglistatin treatment. Data are presented as mean ± SE. p-values (ns, p = 0.33; ****p < 0.0001) were determined using one-way ANOVA followed by Tukey’s multiple comparison test with n = 16 for Ctrl, n = 21 for KLH45 and n = 39 for atglistatin. e, Quantification of LD numbers in astrocytes following KLH45 and atglistatin treatment. Data are presented as mean ± SE. p-values (ns, p = 0.83; **p = 0.01) were determined using one-way ANOVA followed by Tukey’s multiple comparison test with n = 10 for Ctrl, n = 13 for KLH45, and n = 15 for atglistatin.
