Extended Data Fig. 4: Inhibition of IGFBP7 suppressed IGF1R/AKT induced FOXO3a inactivation.
From: Insulin-like growth factor-binding protein-7 (IGFBP7) links senescence to heart failure
(a) Decreased Igf-1 level in Igfbp7-/- mice hearts and serum as measured by Igf-1 Elisa assay 2 weeks post-surgery. n = 6 for heart lysates, and n = 3 for serum. Error bars represent s.e.m., one-way ANOVA with Bonferroni multiple comparisons was used to calculate p values. (b) Representative immunoblot shown Igfbp7 deficiency diminished Igf-1 induced Akt and FoxO3a phosphorylation in mouse neonatal cardiomyocytes (mNCM). Gapdh was used as loading control.
