Fig. 4: Quantification of reactive IgG B-cell clones.

a Type specific and cross-reactive clones targeting SARS-CoV-2 and alpha-CoV or SARS-CoV-2 and beta-CoV pre- and end-pandemic are enumerated and plotted in Venn diagrams for S-trimer, S1, and S2. b The fraction of clones that are SARS-CoV-2-specific (red), sHCoV-specific (blue), or cross-reactive (green) from pooled pre- and pooled end-pandemic donors is plotted. c Proportion of beta-CoV clones, including SARS-CoV-2, that are reactive towards S1 (orange) or S2 (dark red) pre- and end-pandemic. d The level of reactivity of SARS-CoV-2, 229E and NL63 S1-reactive (left panel) as well as SARS-CoV-2, HKU1 and OC43 S2-reactive IgG clones (right panel) detected in end-pandemic donors is indicated as the mean fluorescent intensity (MFI; range: 1,000-65,350). Cross-reactivity of clones is indicated by a connecting line.