In this study, the authors present a cellular assay to probe macrodomain ADP-ribosylhydrolase activity. This relies on PARP15, forming nuclear foci upon automodification, which are dissolved by loss of MARylation. The system enables functional studies and screening of macrodomain and PARP15 inhibitors in physiologically relevant conditions, capturing cell permeability and cytotoxicity.
- Sarah Knapp
- Verena Weber
- Patricia Korn
