Abstract
We have discovered an antifreeze protein1 in an overwintering perennial ryegrass, Lolium perenne. The protein is stable at 100 °C and although it is a less effective antifreeze than proteins found in antarctic fish and insects, it is better at preventing ice recrystallization. This property enables grasses to tolerate ice formation in their tissues without being damaged, suggesting that the control of ice-crystal growth rather than the prevention of freezing may have evolved to be the critical factor in their survival at very low temperatures.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on SpringerLink
- Instant access to the full article PDF.
USD 39.95
Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others
References
Feeney, R. E. & Yeh, Y. Food Technol. 47, 82–88 (1993).
Thomashow, M. F. Adv. Genet. 28, 99–131 ( 1990).
Guy, C. L. Annu. Rev. Plant Physiol. 41, 187– 223 (1990).
Frohman, M. A., Dush, M. K. & Martin, G. R. Proc. Natl Acad. Sci. USA 85, 8998–9002 (1988).
Knight, C. A., Cheng, C. & DeVries, A. L. Biophys. J. 59, 409– 418 (1991).
Arrondo, J. L. R., Young, N. M. & Mantsch, H. H. Biochim. Biophys. Acta 952, 261–268 (1988).
Li, N., Kendrick, B. S., Manning, M. C., Carpenter, J. F. & Duman, J. G. Arch. Biochem. Biophys. 360, 25–32 ( 1998).
Worrall, D. et al. Science 282, 115–117 (1998).
Knight, C. A., DeVries, A. L. & Oolman, L. D. Nature 308, 295– 296 (1984).
Duman, J. G., Wu, D. W., Olsen, T. M., Urrutia, M. & Tursman, D. Adv. Low-Temp. Biol. 2, 131– 182 (1993).
Graham, L. A., Liou, Y.-C, Walker, V. K. & Davies, P. L. Nature 388, 727–728 ( 1997).
Li, N., Andorfer, C. A. & Duman, J. G. J. Exp. Biol. 201, 2243– 2251 (1998).
Hall, D. G & Lips, A. Langmuir 15, 1905–1912 (1999).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Sidebottom, C., Buckley, S., Pudney, P. et al. Heat-stable antifreeze protein from grass . Nature 406, 256 (2000). https://doi.org/10.1038/35018639
Issue date:
DOI: https://doi.org/10.1038/35018639
This article is cited by
-
Physiological Change and Transcriptome Analysis of Chinese Wild Vitis amurensis and Vitis vinifera in Response to Cold Stress
Plant Molecular Biology Reporter (2020)
-
A brief review of applications of antifreeze proteins in cryopreservation and metabolic genetic engineering
3 Biotech (2019)
-
Proteomics: A tool to decipher cold tolerance
Theoretical and Experimental Plant Physiology (2019)
-
Polymer mimics of biomacromolecular antifreezes
Nature Communications (2017)
-
Purification and Identification of Antifreeze Protein From Cold-Acclimated Oat (Avena sativa L.) and the Cryoprotective Activities in Ice Cream
Food and Bioprocess Technology (2016)


