Erratum: Production of gene-targeted sheep by nuclear transfer from cultured somatic cells

Nature 405, 1066— 1069 2000.

An incomplete version of Figure 1 was printed in this Letter. The correct Figure is reproduced below.

Figure 1: Diagrams of ovine COL1A1, COLT-1 and COLT-2 targeting vectors and targeted COL1A1 locus.

The map of ovine COL1A1 shows the translational stop locus. The map of ovine COL1A1 shows the translational stop and polyadenylation sites, the direction of transcription and extent of the COL1A1 mRNA is indicated by an arrow, an asterisk marks an Ssp1 site where the targeted gene insertions were made. Southern hybridization probes and PCR primers are indicated. The maps of the COLT-1 and COLT-2 vectors and the targeted COL1A1 locus show the IRES–neo cassette as a shaded box, the BLG driven AAT transgene by a striped box, and the bacterial vector pSL1180 by an open box. The direction and predicted extent of the COL1A1 / IRES–neo bicistronic mRNA and the AAT transgene mRNA are shown as arrows. The scale bar represents 2 kb. Restriction enzyme sites: A, AspI; B, BamHI; Sc, SacII; S, SalI; K, Kpn I; X, XhoI.