Figure 3
CXCR4-mediated migration of prostate cancer and normal prostatic epithelial cells. PrEC, PC3 and LNCaP cells were tested for their ability to migrate toward chemotactic gradients of 0 (□) or 100 ng/ml of CXCL12 (▧). Similarly, cells were cocultured with 1 μg/ml of monoclonal antibodies against CXCR4 (▪) or IgG2a control antibody (▨) during migration assays with CXCL12. The number of cells (±s.e.m.) that migrated to CXCL12 out of the initial 104 cells used to seed the migration chamber are shown, with asterisks (
) indicating significant differences (P<0.05) between untreated (□) and CXCL12-induced cells (▧, ▨, ▪), while migration indexes below 1 per 104 cells are designated below detection (BD).
