Figure 5
CXCL12-induced collagenase expression by normal prostatic epithelial and prostate carcinoma cells. LNCaP (▪), PC3 (▨) and PrEC (□) cells were tested for their ability to express collagenases (MMP-1 and MMP-13) mRNA and active protein. PrEC, LNCaP and PC3 cells were cultured with and without CXCL12 (100 ng/ml). RNA was isolated, and quantitative RT-PCR analysis was performed (in triplicate) for mRNA expression of collagenases (upper panel) and transcript copies are expressed relative to actual copies of 18S rRNA. Active collagenases were quantified by protein ELISA in conditioned medium (lower panel). MMP expression below the detectable limit of the RT-PCR or ELISA assay are designated as below detection (BD). Asterisk(s) indicate statistically significant differences (P<0.05) between PrEC and prostate cancer cells (
), between untreated and treated cells (
), or between similarly treated prostate cancer cells (★) that produced the highest levels of collagenase.
