Figure 6
CXCL12-induced gelatinase expression by normal prostatic epithelial and prostate cancer cells. LNCaP (▪), PC3 (▨) and PrEC (□) cells were tested for their ability to express gelatinases (MMP-2 and -9). PrEC, LNCaP and PC3 cells were cultured with and without 100 ng/ml of CXCL12. Quantitative RT-PCR analysis was used to measure mRNA expression of gelatinases (upper panel) and transcript copies are expressed relative to actual copies of 18S rRNA. Active gelatinases were quantified by protein ELISA in conditioned medium (lower panel). MMP expression below the detectable limit of the RT-PCR or ELISA assays are designated as below detection (BD). Asterisk(s) indicate statistically significant differences (P<0.05) between PrEC and prostate cancer cells (
), between untreated and treated cells (
), or between similarly treated prostate cancer cells (★) that produced the highest levels of MMP-2 or -9.
