Figure 7
CXCL12-induced stromelysin expression by prostate cancer cells. PrEC (□), LNCaP (▪) and PC3 (▨) cells were tested for their ability to express stromelysin-1 (MMP-3), -2 (MMP-10) and -3 (MMP-11). PrEC, LNCaP and PC3 cells were cultured with and without 100 ng/ml of CXCL12. Quantitative RT-PCR analysis was used to measure mRNA expression of stromelysins (upper panel) and copies of transcripts are expressed relative to actual copies of 18S rRNA. Active MMP was quantified by protein ELISA of culture supernatants (lower panel). MMP expression below the detectable limit of the RT-PCR or ELISA assay are designated as below detection (BD). Asterisk(s) indicate statistically significant differences (P<0.05) between PrEC and prostate cancer cells (
), between untreated and treated cells (
), or between similarly treated prostate cancer cells (★) that produced the highest levels of MMP-3, -10 or -11.
