Figure 4

Scatter plots and 2-D representations of whole sections. (a–c) Representative data from five matched whole sections. (a) scatter plots of normalized AQUA scores of estrogen receptor are shown. Each image obtained on the section is assigned a field number starting in the upper left corner of the slide, across the row and down to the next row in a serpentine pattern. (b) heat maps showing AQUA ER scores assembled into a ‘virtual slide’ are shown. The most intense red are the highest ER scores and the most intense green are the lowest ER scores. (c) Corresponding H&E-stained whole sections used to identify areas of invasive cancers.