Figure 3

Complement-mediated lysis of HeLa and CC8 cells in the presence of anti-mCRP mAb. Experiments were performed as described in “Materials and Methods.” A representative experiment is shown. Amount of lysis is determined as the percentage 51Cr released, after complement activation by anti-HLA Class I mAb (W6/32). Baby rabbit complement (BRC) was used as a positive control for complement activation. Under the x axis, the blocked mCRP are depicted. *Negative control, complement activation in the absence of anti-HLA Class I and in the presence of blocking mAb against all three mCRP.