Figure 7
Comparison of immunohistochemistry and in situ hybridization of tonsil tissues formalin fixed with and without ultrasound. Immunohistochemistry for kappa light chain antigen (a–d) and in situ hybridization for kappa light chain mRNA (e–h). Tonsils fixed for 6 h (b and f) show specific immunohistochemistry staining of kappa light chain antigen but no kappa light chain mRNA signals. Tonsils fixed for 22 h (c and g) show sporadic mRNA staining along the peripheral edges. In all, 15-min ultrasound-fixed tonsils (d and h) demonstrate strong, specific mRNA staining of plasma cells in the germinal center and the peripheral edge, corresponding to immunohistochemistry signals. Original magnification × 20.
